Pooled Populations from the AKF
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These T-DNA pools were donated by Michael Sussman and Rick Amasino at the Arabidopsis Knockout Facility (AKF).
Plasmid Construct
The construct, introduced into Arabidopsis ecotype Columbia (Col) via Agrobacterium vacuum infiltration, is called pD991-AP3. It is a derivative of the pD991, vector from Dr. Thomas Jack as shown Figure 1 (below).
Figure 1: T-DNA construct used for the AKF Pools (click to enlarge). LB and RB, left and right borders, respectively; NPTII, kanamycin resistance gene for selection in bacteria.
Selectable Marker
As seen in Figure 1, the positive selectable marker is the NPTII (kanamycin resistance) gene which is driven by 35S promoter.
Sequence data for the portion of the vector that is transferred into plants can be found by clicking here.
Primer Sequences
For information on the primer sequences for inverse and TAIL PCR, click here.
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