All the information contained on this page can be found under the Mutation Section at the John Innes Centre’s Genome Laboratory Website. The Muttion Section site can be accessed by clicking here.

The Enhancer Trap collection was produced by Dr. Jonathan Clarke and Prof. Mike Bevan at the John Innes Centre.

Click here to download a PDF which document describes the genotyping / insertion site verification protocol for Enhancer Trap Insert Lines. The document defines the transposon specific primer used and provides a model for genotyping your insertions with insertion site specific primers.

For information on Enhancer Trap technology and information on the construct and starter lines, click here to download a second PDF document. This PDF also describes the protocols that are used to isolate insertion sites and to exploit the Enhancer Trap lines for functional genomics.

AT Line	GT Line