The GABI-Kat T-DNA Insertion lines were generated by Prof. Weisshaar and colleagues at the Max Planck Institute for Plant Breeding Research in Germany.

Plasmid Construct and Selectable Marker

Two binary vectors, pAC106 and pAC161, designed for activation tagging were used to generate the T-DNA mutagenised population. Both vectors carry the SUL ORF gene for resistance against the herbicide sulfadiazine which allows for the selection of transformed plants in the greenhouse.

Vector pAC106 carries the two full-length 35S CaMV promoters. One is located at the T-DNA right border, while the second drives expression of synthetic SUL ORF. Vector pAC161 (see Figure 1 below) carries the 1'-2' promoter that drives the expression of the SUL ORF and replaces one of the two 35S CaMV promoters of pAC106.

Figure 1: Plasmid map of pAC161 mainly used for transformation. Map of the binary transformation vector pAC161 that contains the SULf ORF driven by the 1'-2' promoter. The 35S CaMV promoter located at the right border can act as an activation tagging element after T-DNA integration. Structural parts and relevant restriction sites of the vector are marked.

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For information on the sequencing strategy and primer sequences, click here.

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