All the information contained on this page can be found under the Mutation Section at the John Innes Centre’s Genome Laboratory Website. The Muttion Section site can be accessed by clicking here.

The Gene Trap collection was produced at the John Innes Centre.

Click here to download a PDF which document describes the genotyping/insertion site verification protocol for Gene Trap Insert transposon lines. The document defines the transposon specific primer used and provides a model for genotyping your insertions with insertion site specific primers.

For information on Gene Trap technology and information on the construct and starter lines, click here to download a second PDF document. This PDF also describes the protocols that are used to isolate insertion sites and to exploit the Gene Trap lines for functional genomics.

Also visit the NASC Gene Trap page for more information.

ET Line	SLAT Line