The SAIL (Syngenta Arabidopsis Insertion Library) insertion lines were generated in the United States at three locations: the Torrey Mesa Research Institute in California; Syngenta Biotechnology Incorporated in North Carolina; and Syngenta Seeds Incorporated in California.

Plasmid Construct and Selectable Markers

Two different binary vectors were used to generate the SAIL populations. The T-DNA in pCSA110 is 7541 kb in length and contains, from the left to right border, a BASTA resistance cassette, pBluescript SKII+, and a Lat52 promoter-ß-glucuronidase fusion. The T-DNA in pDAP101 is 4763 kb in length and contains, from the left to right border, a BASTA resistance cassette and a pBluescript SKII+. Both T-DNAs have the same left border but different right borders.

Mapping of T-DNA Insertion Sites

Fragments flanking the T-DNA inserts can be amplified using thermal asymmetric interlaced (TAIL) PCR (as described by Liu et. al., 1995) with each rounds of amplification with the following primers:

The left border (both T-DNA have the same left border sequence):

LB1 (5’-GCC TTT TCA GAA ATG GAT AAA TAG CCT TGC TTC C-3’)

LB2 (5’-GCT TCC TAT TAT ATC TTC CCA AAT TAC CAA TAC A-3’)

LB3 (5’-TAG CAT CTG AAT TTC ATA ACC AAT CTC GAT ACA C-3’)

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For the right border sequence in pCSA110:

qRB1 (5’-CAA ACT AGG ATA AAT TAT CGC GCG CGG TGT C-3’)

qRB2 (5’-GGT GTC ATC TAT GTT ACT AGA TCG GGA ATT GA-3’)

qRB3 (5’-CGC CAT GGC ATA TGC TAG CAT GCA TAA TTC-3’)

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For the right border sequence in pDAP101:

RB1 (5’-ATT AGG CAC CCC AGG CTT TAC ACT TTA TG-3’)

RB2 (5’-GTA TGT TGT GTG GAA TTG TGA GCG GAT AAC-3’)

RB3 (5’-TAA CAA TTT CAC ACA GGA AAC AGC TAT AC-3’)

RB3 (5’-TAA CAA TTT CAC ACA GGA AAC AGC TAT AC-3’)

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SM Line	SALK Line