The SALK Population at NASC are single, segregating flank-tagged T3-generation T-DNA lines. The population was generated by Dr. Joseph Ecker, at the The Salk Institute in California, USA.

Plasmid Construct

The construct that has been introduced into Arabidopsis ecotype Columbia (Col) via Agrobacterium tumefaciens vacuum infiltration is the pROK2 vector, a derivative of the pBIN19 vector, as shown in Figure 1 (below).

Figure 1: T-DNA construct used for SALK Lines.LB and RB, left and right borders, respectively; NPTII, kanamycin resistance gene for selection in bacteria.

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Selectable Marker

As seen in Figure 1, the selectable marker is the NPTII (kanamycin resistance) gene. However, after several generation of growth, some of the lines show silencing of this gene. Thus, it is not unusual for a mutant line to not express the drug resistance phenotype.

Primer Sequences

For information on the primer sequences for inverse and TAIL PCR, click here.

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SAIL Line	AKF Line